NAD⁺ Synthetase is Required for Free-living and Symbiotic Nitrogen Fixation in the Actinobacterium Frankia casuarinae.

ABSTRACT: Frankia spp. are multicellular actinobacteria that fix atmospheric dinitrogen (N₂) not only in the free-living state, but also in root-nodule symbioses with more than 200 plant species, called actinorhizal plants. To identify novel Frankia genes involved in N₂ fixation, we previously isolated mutants of Frankia casuarinae that cannot fix N₂. One of these genes, mutant N3H4, did not induce nodulation when inoculated into the host plant Casuarina glauca. Cell lineages that regained the ability to fix N₂ as free-living cells were isolated from the mutant cell population. These restored strains also regained the ability to stimulate nodulation. A comparative ana-lysis of the genomes of mutant N3H4 and restored strains revealed that the mutant carried

SUBMITTER: Kucho KI 

PROVIDER: S-EPMC10037102 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature