Identifying and Overcoming Artifacts in ¹ H-Based Saturation Transfer NOE NMR Experiments.

ABSTRACT: Magnetization transfer experiments are versatile nuclear magnetic resonance (NMR) tools providing site-specific information. We have recently discussed how saturation magnetization transfer (SMT) experiments could leverage repeated repolarizations arising from exchanges between labile and water protons to enhance connectivities revealed via the nuclear Overhauser effect (NOE). Repeated experience with SMT has shown that a number of artifacts may arise in these experiments, which may confound the information being sought - particularly when seeking small NOEs among closely spaced resonances. One of these pertains to what we refer to as "spill-over" effects, originating from the use of long saturation pulses leading to changes in the signals of proximate peaks. A second, related but in fact

SUBMITTER: Grun JT 

PROVIDER: S-EPMC10037324 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature