Nanopore Detection Using Supercharged Polypeptide Molecular Carriers.

ABSTRACT: The analysis at the single-molecule level of proteins and their interactions can provide critical information for understanding biological processes and diseases, particularly for proteins present in biological samples with low copy numbers. Nanopore sensing is an analytical technique that allows label-free detection of single proteins in solution and is ideally suited to applications, such as studying protein-protein interactions, biomarker screening, drug discovery, and even protein sequencing. However, given the current spatiotemporal limitations in protein nanopore sensing, challenges remain in controlling protein translocation through a nanopore and relating protein structures and functions with nanopore readouts. Here, we demonstrate that supercharged unstructured polypeptides (SUPs)

SUBMITTER: Wang X 

PROVIDER: S-EPMC10037339 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature