Hexafluoroisopropanol-based deep eutectic solvents for high-performance DNA extraction.

ABSTRACT: In this study, hexafluoroisopropanol (HFIP)-based deep eutectic solvents (DESs) were developed and used for DNA extraction from human whole blood samples for the first time. HFIP-based DESs were prepared using HFIP and choline chloride (ChCl)/tetrabutylammonium chloride/cetyltrimethylammonium bromide as the hydrogen bond donor and acceptor, respectively. The two-phase system formation was promoted with different inorganic salts as the phase-forming component. According to the strong phase separation capability and high DNA extraction efficiency, DESs consisting of HFIP/ChCl-(NH₄)₂SO₄, HFIP/ChCl-Na₂SO₄ and HFIP/ChCl-MgSO₄ were then selected for application in DNA extraction. The factors that could have impacted the DNA extrac

SUBMITTER: Xu J 

PROVIDER: S-EPMC10037425 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature