Evaluating the sampling effort for the metabarcoding-based detection of fish environmental DNA in the open ocean.

ABSTRACT: Clarifying the effect of the sampling protocol on the detection of environmental DNA (eDNA) is essential for appropriately designing biodiversity research. However, technical issues influencing eDNA detection in the open ocean, which consists of water masses with varying environmental conditions, have not been thoroughly investigated. This study evaluated the sampling effort for the metabarcoding-based detection of fish eDNA using replicate sampling with filters of different pore sizes (0.22 and 0.45 μm) in the subtropical and subarctic northwestern Pacific Ocean and Arctic Chukchi Sea. The asymptotic analysis predicted that the accumulation curves for detected taxa did not saturate in most cases, indicating that our sampling effort (7 or 8 replicates, corresponding to 10.5-40 L of filtrat

SUBMITTER: Kawakami T 

PROVIDER: S-EPMC10037434 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature