Metformin regulates expression of DNA methyltransferases through the miR-148/-152 family in non-small lung cancer cells.

ABSTRACT:

Background

To understand the molecular mechanisms involved in regulation of DNA methyltransferases (DNMTs) by metformin in non-small cell lung cancer (NSCLC) cells.

Methods

Expression levels of DNMTs in response to metformin were analyzed in NSCLC cells. MicroRNAs regulating expression of DNMTs at the post-transcriptional level were searched using miRNA-target databases (miRDB and miRTarBase), TCGA RNASeqV2 lung cancer data, and miRNA-seq.

Results

Metformin dose-dependently downregulated expression of DNMT1 and DNMT3a at the post-transcriptional level and expression of DNMT3b at the transcriptional level in A549 lung cancer cells. Activity of DNMTs was reduced by about 2.6-fold in A549 cells treated with 10 mM metformin for 72 h. miR-148/-152 family members (miR-148a,

SUBMITTER: Lee BB 

PROVIDER: S-EPMC10037810 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature