Ratiometric electrochemical OR gate assay for NSCLC-derived exosomes.

ABSTRACT: Non-small cell lung cancer (NSCLC) is the most common pathological type of LC and ranks as the leading cause of cancer deaths. Circulating exosomes have emerged as a valuable biomarker for the diagnosis of NSCLC, while the performance of current electrochemical assays for exosome detection is constrained by unsatisfactory sensitivity and specificity. Here we integrated a ratiometric biosensor with an OR logic gate to form an assay for surface protein profiling of exosomes from clinical serum samples. By using the specific aptamers for recognition of clinically validated biomarkers (EpCAM and CEA), the assay enabled ultrasensitive detection of trace levels of NSCLC-derived exosomes in complex serum samples (15.1 particles μL^-1 within a linear range of 10²-10⁸

SUBMITTER: Meng F 

PROVIDER: S-EPMC10037838 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature