IreK-Mediated, Cell Wall-Protective Phosphorylation in Enterococcus faecalis.

ABSTRACT: Enterococcus faecalis is a Gram-positive bacterium that is a major cause of hospital-acquired infections due, in part, to its intrinsic resistance to cell wall-active antimicrobials. One critical determinant of this resistance is the transmembrane kinase IreK, which belongs to the penicillin-binding protein and serine/threonine kinase-associated kinase family of bacterial signaling proteins involved with the regulation of cell wall homeostasis. The activity of IreK is enhanced in response to cell wall stress, but direct substrates of IreK phosphorylation, leading to antimicrobial resistance, are largely unknown. To better understand stress-modulated phosphorylation events contributing to antimicrobial resistance, wild type E. faecalis cells treated with cell wall-active antim

SUBMITTER: Iannetta AA 

PROVIDER: S-EPMC10037947 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature