Extracellularly Detectable Electrochemical Signals of Living Cells Originate from Metabolic Reactions.

ABSTRACT: Direct detection of cellular redox signals has shown immense potential as a novel living cell analysis tool. However, the origin of such signals remains unknown, which hinders the widespread use of electrochemical methods for cellular research. In this study, the authors found that intracellular metabolic pathways that generate adenosine triphosphate (ATP) are the main contributors to extracellularly detectable electrochemical signals. This is achieved through the detection of living cells (4,706 cells/chip, linearity: 0.985) at a linear range of 7,466-48,866. Based on this discovery, the authors demonstrated that the cellular signals detected by differential pulse voltammetry (DPV) can be rapidly amplified with a developed medium containing metabolic activator cocktails (MACs). The DPV ap

SUBMITTER: Koo KM 

PROVIDER: S-EPMC10037963 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature