An essential endoplasmic reticulum-resident N-acetyltransferase ortholog in Plasmodium falciparum.

ABSTRACT: N-terminal acetylation is a common eukaryotic protein modification that involves the addition of an acetyl group to the N-terminus of a polypeptide. This modification is largely performed by cytosolic N-terminal acetyltransferases (NATs). Most associate with the ribosome, acetylating nascent polypeptides co-translationally. In the malaria parasite Plasmodium falciparum, exported effectors are thought to be translated into the endoplasmic reticulum (ER), processed by the aspartic protease plasmepsin V and then N-acetylated, despite having no clear access to cytosolic NATs. Here, we used inducible gene deletion and post-transcriptional knockdown to investigate the primary ER-resident NAT candidate, Pf3D7_1437000. We found that it localizes to the ER and is required for parasite growth. Howev

SUBMITTER: Polino AJ 

PROVIDER: S-EPMC10038149 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature