Label-free structural and functional volumetric imaging by dual-modality optical-Raman projection tomography.

ABSTRACT: Mesoscale volumetric imaging is of great importance for the study of bio-organisms. Among others, optical projection tomography provides unprecedented structural details of specimens, but it requires fluorescence label for chemical targeting. Raman spectroscopic imaging is able to identify chemical components in a label-free manner but lacks microstructure. Here, we present a dual-modality optical-Raman projection tomography (ORPT) technology, which enables label-free three-dimensional imaging of microstructures and components of millimeter-sized samples with a micron-level spatial resolution on the same device. We validate the feasibility of our ORPT system using images of polystyrene beads in a volume, followed by detecting biomolecules of zebrafish and Arabidopsis, demonstrating

SUBMITTER: Wang N 

PROVIDER: S-EPMC10038343 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature