Mechanosensitive pore opening of a prokaryotic voltage-gated sodium channel.

ABSTRACT: Voltage-gated ion channels (VGICs) orchestrate electrical activities that drive mechanical functions in contractile tissues such as the heart and gut. In turn, contractions change membrane tension and impact ion channels. VGICs are mechanosensitive, but the mechanisms of mechanosensitivity remain poorly understood. Here, we leverage the relative simplicity of NaChBac, a prokaryotic voltage-gated sodium channel from Bacillus halodurans, to investigate mechanosensitivity. In whole-cell experiments on heterologously transfected HEK293 cells, shear stress reversibly altered the kinetic properties of NaChBac and increased its maximum current, comparably to the mechanosensitive eukaryotic sodium channel Na_V1.5. In single-channel experiments, patch suction reversibly increased t

SUBMITTER: Strege PR 

PROVIDER: S-EPMC10038658 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature