Identification of vitamin B₁₂ producing bacteria based on the presence of bluB/cobT2 homologues.

ABSTRACT:

Objectives

The objective of the study was to develop a strategy for the identification of new vitamin B₁₂-producing species and to characterize their production capability using a fast and sensitive LC-MS/MS method developed in this study.

Results

Searching for homologues of the bluB/cobT2 fusion gene known to be responsible for the production of the active vitamin B₁₂ form in P. freudenreichii was shown to be a successful strategy for the identification of new vitamin B₁₂-producing strains. The analysis of the identified strains via LC-MS/MS showed the ability of Terrabacter sp. DSM102553, Yimella lutea DSM19828 and Calidifontibacter indicus DSM22967 to produce the active form of vitamin B₁₂. Further analysis of vitamin B_12</

SUBMITTER: Dudko D 

PROVIDER: S-EPMC10038948 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature