Study of FOXO1-interacting proteins using TurboID-based proximity labeling technology.

ABSTRACT:

Background

Protein‒protein interactions (PPIs) are the foundation of the life activities of cells. TurboID is a biotin ligase with higher catalytic efficiency than BioID or APEX that reduces the required labeling time from 18 h to 10 min. Since many proteins participate in binding and catalytic events that are very short-lived, it is theoretically possible to find relatively novel binding proteins using the TurboID technique. Cell proliferation, apoptosis, autophagy, oxidative stress and metabolic disorders underlie many diseases, and forkhead box transcription factor 1 (FOXO1) plays a key role in these physiological and pathological processes.

Results

The FOXO1-TurboID fusion gene was transfected into U251 astrocytes, and a cell line stably expressing FOXO1 was constructed.

SUBMITTER: Su Y 

PROVIDER: S-EPMC10039511 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature