A novel feedback regulated loop of circRRM2-IGF2BP1-MYC promotes breast cancer metastasis.

ABSTRACT:

Background

Metastasis is the leading cause of mortality in patients with breast cancer (BC). Studies demonstrate that circular RNAs (circRNAs) were involved in BC progression, while the molecular mechanisms remain largely unclear.

Methods

The microArray circRNA profiles were used to explore the differential expression circRNAs in BC and paracancerous normal tissues, and the quantitative reverse transcription-polymerase chain reaction was used to validate their expression level in clinical samples and cell lines. Nuclear/cytosolic fractionation and fluorescence in situ hybridization (FISH) assays were performed to examine circRRM2 (hsa_circ_0052582) subcellular location. The scratch wound healing and transwell assays were conducted to evaluate the impact of circRRM2 on BC cel

SUBMITTER: Hao R 

PROVIDER: S-EPMC10039515 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature