Cerebral Aβ deposition precedes reduced cerebrospinal fluid and serum Aβ42/Aβ40 ratios in the App^NL-F/NL-F knock-in mouse model of Alzheimer's disease.

ABSTRACT:

Background

Aβ42/Aβ40 ratios in cerebrospinal fluid (CSF) and blood are reduced in preclinical Alzheimer's disease (AD), but their temporal and correlative relationship with cerebral Aβ pathology at this early disease stage is not well understood. In the present study, we aim to investigate such relationships using App knock-in mouse models of preclinical AD.

Methods

CSF, serum, and brain tissue were collected from 3- to 18-month-old App^NL-F/NL-F knock-in mice (n = 48) and 2-18-month-old App^NL/NL knock-in mice (n = 35). The concentrations of Aβ42 and Aβ40 in CSF and serum were measured using Single molecule array (Simoa) immunoassays. Cerebral Aβ plaque burden was assessed in brain tissue sections by immunohistochemistry and thioflavin S staining. Furthe

SUBMITTER: Andersson E 

PROVIDER: S-EPMC10039589 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature