Variability of fluorescence intensity distribution measured by flow cytometry is influenced by cell size and cell cycle progression.

ABSTRACT: The distribution of fluorescence signals measured with flow cytometry can be influenced by several factors, including qualitative and quantitative properties of the used fluorochromes, optical properties of the detection system, as well as the variability within the analyzed cell population itself. Most of the single cell samples prepared from in vitrocultures or clinical specimens contain a variable cell cycle component. Cell cycle, together with changes in the cell size, are two of the factors that alter the functional properties of analyzed cells and thus affect the interpretation of obtained results. Here, we describe the association between cell cycle status and cell size, and the variability in the distribution of fluorescence intensity as determined with flow cytometry, at populatio

SUBMITTER: Fedr R 

PROVIDER: S-EPMC10039904 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature