PHF6 recruits BPTF to promote HIF-dependent pathway and progression in YAP-high breast cancer.

ABSTRACT:

Background

Aberrant epigenetic remodeling events contribute to progression and metastasis of breast cancer (Bca). The specific mechanims that epigenetic factors rely on to mediate tumor aggressiveness remain unclear. We aimed to elucidate the roles of epigenetic protein PHF6 in breast tumorigenesis.

Methods

Published datasets and tissue samples with PHF6 staining were used to investigate the clinical relevance of PHF6 in Bca. CCK-8, clony formation assays were used to assess cell growth capacity. Cell migration and invasion abilities were measured by Transwell assay. The gene mRNA and protein levels were measured by quantitative real-time PCR and western blot. Chromatin immunoprecipitation (ChIP)-qPCR assays were used to investigate transcriptional relationships among genes.

SUBMITTER: Gao S 

PROVIDER: S-EPMC10040131 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature