CPT1A in AgRP neurons is required for sex-dependent regulation of feeding and thirst.

ABSTRACT:

Background

Fatty acid metabolism in the hypothalamus has an important role in food intake, but its specific role in AgRP neurons is poorly understood. Here, we examined whether carnitinea palmitoyltransferase 1A (CPT1A), a key enzyme in mitochondrial fatty acid oxidation, affects energy balance.

Methods

To obtain Cpt1aKO mice and their control littermates, Cpt1a^(flox/flox) mice were crossed with tamoxifen-inducible AgRP^CreERT2 mice. Food intake and body weight were analyzed weekly in both males and females. At 12 weeks of age, metabolic flexibility was determined by ghrelin-induced food intake and fasting-refeeding satiety tests. Energy expenditure was analyzed by calorimetric system and thermogenic activity of brown adipose tissue. To study fluid balan

SUBMITTER: Zagmutt S 

PROVIDER: S-EPMC10040140 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature