Live-Cell Profiling of Penicillin-Binding Protein Inhibitors in Escherichia coli MG1655.

ABSTRACT: Penicillin-binding proteins (PBPs) make up an essential class of bacterial enzymes that carry out the final steps of peptidoglycan synthesis and regulate the recycling of this polymeric structure. PBPs are an excellent drug target and have been the most clinically relevant antibacterial target since the 1940s with the introduction of β-lactams. Despite this, a large gap in knowledge remains regarding the individual function and regulation of each PBP homologue in most bacteria. This can be attributed to a lack of chemical tools and methods that enable the study of individual PBPs in an activity-dependent manner and in their native environment. The development of such methods in Gram-negative bacteria has been particularly challenging due to the presence of an outer membrane and numerous re

SUBMITTER: Shirley JD 

PROVIDER: S-EPMC10040144 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature