Circ_0003340 regulates the expression of ENAH to affect the development of esophageal cancer through miR-874-3p.

ABSTRACT:

Background

Esophageal cancer is a malignant tumor with a poor prognosis and high incidence. Circular RNAs (circRNAs) have been shown to be involved in the pathogenesis of cancers, including esophageal cancer. Here, we explored the precise role of circ_0003340 in esophageal cancer development.

Methods

The expression levels of circ_0003340, miR-874-3p and enabled homolog (ENAH) were detected by quantitative real-time polymerase chain reaction and western blot. Subcellular localization and RNase R assays were used to characterize circ_0003340. Cell Counting Kit 8, flow cytometry, transwell assays were used to analyze cell proliferation, apoptosis, migration and invasion. The effect of circ_0003340 on tumor growth was assessed by tumor experiments in vivo. Dual-luciferase report

SUBMITTER: Wang J 

PROVIDER: S-EPMC10040281 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature