Generation and characterization of humanized affinity-matured EGFL6 antibodies for ovarian cancer therapy.

ABSTRACT:

Objectives

Epidermal growth factor EGF-like domain multiple-6 (EGFL6) is highly expressed in high grade serous ovarian cancer and promotes both endothelial cell proliferation/angiogenesis and cancer cell proliferation/metastasis. As such it has been implicated as a therapeutic target. As a secreted factor, EGFL6 is a candidate for antibody therapy. The objectives of this study were to create and validate humanized affinity-matured EGFL6 neutralizing antibodies for clinical development.

Methods

A selected murine EGFL6 antibody was humanized using CDR grafting to create 26 variant humanized antibodies. These were screened and the lead candidate was affinity matured. Seven humanized affinity-matured EGFL6 antibodies were screened for their ability to block EGFL6 activity on can

SUBMITTER: Tang H 

PROVIDER: S-EPMC10040429 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature