Proteomic characterisation of perhexiline treatment on THP-1 M1 macrophage differentiation.

ABSTRACT:

Background

Dysregulated inflammation is important in the pathogenesis of many diseases including cancer, allergy, and autoimmunity. Macrophage activation and polarisation are commonly involved in the initiation, maintenance and resolution of inflammation. Perhexiline (PHX), an antianginal drug, has been suggested to modulate macrophage function, but the molecular effects of PHX on macrophages are unknown. In this study we investigated the effect of PHX treatment on macrophage activation and polarization and reveal the underlying proteomic changes induced.

Methods

We used an established protocol to differentiate human THP-1 monocytes into M1 or M2 macrophages involving three distinct, sequential stages (priming, rest, and differentiation). We examined the effect of PHX treatm

SUBMITTER: Dhakal B 

PROVIDER: S-EPMC10040681 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature