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Facile electrochemical affinity measurements of small and large molecules.

ABSTRACT: A novel miniaturized sensor for electrochemical detection that contains graphene- and gold nanoparticles was functionalized with proteins. Using cyclic voltammetry (CV) and differential pulse voltammetry (DPV) it was possible to observe and quantify interactions of molecules with these proteins. The protein binders included carbohydrate ligands as small as carbohydrates up to COVID-19 spike protein variants engaged in protein-protein interactions. The system uses off-the-shelf sensors combined with an affordable potentiostat and yet is sensitive enough for small ligand binding.

SUBMITTER: Zaree P

PROVIDER: S-EPMC10041147 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature

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Facile electrochemical affinity measurements of small and large molecules.

Zaree Pouya P Tomris Ilhan I de Vos Sander D SD van der Woude Roosmarijn R Flesch Frits M FM Klein Gebbink Robertus J M RJM de Vries Robert P RP Pieters Roland J RJ

RSC advances 20230327 14

A novel miniaturized sensor for electrochemical detection that contains graphene- and gold nanoparticles was functionalized with proteins. Using cyclic voltammetry (CV) and differential pulse voltammetry (DPV) it was possible to observe and quantify interactions of molecules with these proteins. The protein binders included carbohydrate ligands as small as carbohydrates up to COVID-19 spike protein variants engaged in protein-protein interactions. The system uses off-the-shelf sensors combined w ...[more]

PMID: 36994086

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