Dynamic loading leads to increased metabolic activity and spatial redistribution of viable cell density in nucleus pulposus tissue.

ABSTRACT:

Background

Nucleus pulposus (NP) cell density is orchestrated by an interplay between nutrient supply and metabolite accumulation. Physiological loading is essential for tissue homeostasis. However, dynamic loading is also believed to increase metabolic activity and could thereby interfere with cell density regulation and regenerative strategies. The aim of this study was to determine whether dynamic loading could reduce the NP cell density by interacting with its energy metabolism.

Methods

Bovine NP explants were cultured in a novel NP bioreactor with and without dynamic loading in milieus mimicking the pathophysiological or physiological NP environment. The extracellular content was evaluated biochemically and by Alcian Blue staining. Metabolic activity was determined by m

SUBMITTER: Salzer E 

PROVIDER: S-EPMC10041377 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature