Specific Detection of Physiological S129 Phosphorylated α-Synuclein in Tissue Using Proximity Ligation Assay.

ABSTRACT:

Background

Synucleinopathies are a group of neurodegenerative disorders that are pathologically characterized by intracellular aggregates called Lewy bodies. Lewy bodies are primarily composed of α-synuclein (asyn) protein, which is mostly phosphorylated at serine 129 (pS129) when aggregated and therefore used as a marker for pathology. Currently commercial antibodies against pS129 asyn stain aggregates well but in healthy brains cross react with other proteins, thus making it difficult to specifically detect physiological pS129 asyn.

Objective

To develop a staining procedure that detects endogenous and physiological relevant pS129 asyn with high specificity and low background.

Methods

We used the fluorescent and brightfield in situ proximity ligation assay (PLA) to s

SUBMITTER: Arlinghaus R 

PROVIDER: S-EPMC10041430 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature