Process intensification for lentiviral vector manufacturing using tangential flow depth filtration.

ABSTRACT: For gene therapies to become more accessible and affordable treatment options, process intensification is one possible strategy to increase the number of doses generated per batch of viral vector. Process intensification for lentiviral vector manufacturing can be achieved by enabling perfusion in the production bioreactor when applied in tandem with a stable producer cell line, allowing for significant expansion of cells and production of lentiviral vectors without the need for transfer plasmids. Tangential flow depth filtration was used to achieve an intensified lentiviral vector production by enabling perfusion to expand cell density and allow for continuous separation of lentiviral vectors from producer cells. Hollow-fiber depth filters made of polypropylene with 2- to 4-μm channels dem

SUBMITTER: Tona RM 

PROVIDER: S-EPMC10041461 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature