FUT2 inhibits the EMT and metastasis of colorectal cancer by increasing LRP1 fucosylation.

ABSTRACT:

Background

Fucosyltransferase 2(FUT2) and its induced α-1,2 fucosylation is associated with cancer metastasis. However, the role of FUT2 in colorectal cancer (CRC) metastasis remains unclear.

Methods

The expression levels and clinical analyses of FUT2 were assessed in CRC samples. Migration and invasion assays, EMT detection, nude mice peritoneal dissemination models and intestinal specific FUT2 knockout mice (FUT2^△IEC mice) were used to investigate the effect of FUT2 on metastasis in colorectal cancer. Quantitative proteomics study of glycosylated protein, UEA enrichment, Co-immunoprecipitation identified the mediator of the invasive-inhibiting effects of FUT2.

Results

FUT2 is downregulated in CRC tissues and is positively correlated with the survival of C

SUBMITTER: He L 

PROVIDER: S-EPMC10041739 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature