N⁶-methyladenosine reader YTHDF1 regulates the proliferation and migration of airway smooth muscle cells through m⁶A/cyclin D1 in asthma.

ABSTRACT: Asthma is a chronic inflammatory respiratory disease, which is involved in multiple pathologic molecular mechanisms and presents a huge challenge to clinic nursing. Emerging evidence suggests that N⁶-methyladenosine (m⁶A) plays critical roles in respiratory system disease. Thus, present work tried to investigate the functions of m⁶A reader YTHDF 1 in asthma. The results indicated that YTHDF1 significantly upregulated in platelet-derived growth factor (PDGF) induced airway smooth muscle cells (ASMCs). Functionally, overexpression of YTHDF1 promoted the proliferation and migration of ASMCs, while YTHDF1 knockdown repressed the proliferation and migration. Mechanistically, there was a m⁶A modification site on cyclin D1 RNA (CCND1 genome) and YTHDF1

SUBMITTER: Wang J 

PROVIDER: S-EPMC10042154 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature