Understanding the Impact of Protein-Excipient Interactions on Physical Stability of Spray-Dried Protein Solids.

ABSTRACT: Mannitol, leucine, and trehalose have been widely used in spray-dried formulations, especially for inhalation formulations. The individual contribution of these excipients on protein physical stability in spray-dried solids was studied here using bovine serum albumin (BSA) as a model protein. The spray-dried solids were characterized with scanning electron microscopy, powder X-ray diffraction, and solid-state Fourier-transform infrared spectroscopy to analyze particle morphology, crystallinity, and secondary structure change, respectively. Advanced solid-state characterizations were conducted with solid-state hydrogen-deuterium exchange (ssHDX) and solid-state nuclear magnetic resonance (ssNMR) to explore protein conformation and molecular interactions in the context of the system physical

SUBMITTER: Chen Y 

PROVIDER: S-EPMC10042268 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature