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Blood parasite load by qPCR as therapeutic monitoring in visceral leishmaniasis patients in Brazil: a case series study.

ABSTRACT:

Background

This study aimed to describe the kinetics of Leishmania parasite load determined using kinetoplast DNA (kDNA)-based quantitative polymerase chain reaction (qPCR) in visceral leishmaniasis (VL) patients.

Methods

Parasite load in blood was assessed by qPCR at five time points, up to 12 months post-diagnosis. Sixteen patients were followed up.

Results

A significant reduction in the parasite load was observed after treatment (P < 0.0001). One patient had an increased parasite load 3 months post-treatment and relapsed clinically at month six.

Conclusions

We have described the use of kDNA-based qPCR in the post-treatment follow-up of VL cases.

SUBMITTER: Aquino SR

PROVIDER: S-EPMC10042474 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature

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Blood parasite load by qPCR as therapeutic monitoring in visceral leishmaniasis patients in Brazil: a case series study.

Aquino Samuel Ricarte de SR Diniz Lucyo Flávio Bezerra LFB Nunes Sávio Luiz Pereira SLP Silva Roberta Lane de Oliveira RLO Gouveia Gisele Veneroni GV Gouveia João José Simoni JJS Sales Kamila Gaudêncio da Silva KGDS Dantas-Torres Filipe F Carmo Rodrigo Feliciano do RFD

Revista da Sociedade Brasileira de Medicina Tropical 20230327

<h4>Background</h4>This study aimed to describe the kinetics of Leishmania parasite load determined using kinetoplast DNA (kDNA)-based quantitative polymerase chain reaction (qPCR) in visceral leishmaniasis (VL) patients.<h4>Methods</h4>Parasite load in blood was assessed by qPCR at five time points, up to 12 months post-diagnosis. Sixteen patients were followed up.<h4>Results</h4>A significant reduction in the parasite load was observed after treatment (P < 0.0001). One patient had an increased ...[more]

PMID: 36995785

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Low parasite load estimated by qPCR in a cohort of children living in urban area endemic for visceral leishmaniasis in Brazil.

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