Optic nerve injury models under varying forces.

ABSTRACT:

Purpose

To explore the pathological changes in optic nerve injury models under varying forces.

Methods

The rats were classified into 4 groups: sham operation (SH), 0.1, 0.3, and 0.5 N. Modeling was performed using the lateral optic nerve pulling method. Seven days after modeling, Brn3a immunofluorescence was used to detect retinal ganglion cell (RGC) number, terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining was used to detect RGC apoptosis, and flash visual evoked potential (FVEP) was used to detect the optic nerve function on days 1, 3, and 7 after modeling. In addition, LC3 II and P62 expression levels in retinal tissues were detected by western blotting to observe the changes in autophagy levels.

Results

RGC number decreased 7 d after mo

SUBMITTER: Sun W 

PROVIDER: S-EPMC10042766 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature