Single-molecule localization microscopy reveals the ultrastructural constitution of distal appendages in expanded mammalian centrioles.

ABSTRACT: Distal appendages (DAPs) are vital in cilia formation, mediating vesicular and ciliary docking to the plasma membrane during early ciliogenesis. Although numerous DAP proteins arranging a nine-fold symmetry have been studied using superresolution microscopy analyses, the extensive ultrastructural understanding of the DAP structure developing from the centriole wall remains elusive owing to insufficient resolution. Here, we proposed a pragmatic imaging strategy for two-color single-molecule localization microscopy of expanded mammalian DAP. Importantly, our imaging workflow enables us to push the resolution limit of a light microscope well close to a molecular level, thus achieving an unprecedented mapping resolution inside intact cells. Upon this workflow, we unravel the ultra-resolved hig

SUBMITTER: Chang TB 

PROVIDER: S-EPMC10043031 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature