An impact of age on respiratory syncytial virus infection in air-liquid-interface culture bronchial epithelium.

ABSTRACT:

Background

Elderly people are known to be vulnerable to virus infection. However, this has not been appropriately tested in in vitro studies due to a lack of appropriate virus infection models. In this report, we investigated the impact of age on respiratory syncytial virus (RSV) in pseudostratified air-liquid-interface (ALI) culture bronchial epithelium, which more closely mimic human airway epithelium morphologically and physiologically, than submerged cancer cell line cultures.

Methods

RSV A2 was inoculated apically to the bronchial epithelium obtained from 8 donors with different ages (28-72 years old), and time-profiles of viral load and inflammatory cytokines were analyzed.

Results

RSV A2 replicated well in ALI-culture bronchial epithelium. The viral peak

SUBMITTER: Ito K 

PROVIDER: S-EPMC10043235 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature