α-Pinene: Docking Study, Cytotoxicity, Mechanism of Action, and Anti-Biofilm Effect against Candida albicans.

ABSTRACT: Candida albicans is associated with serious infections in immunocompromised patients. Terpenes are natural-product derivatives, widely studied as antifungal alternatives. In a previous study reported by our group, the antifungal activity of α-pinene against C. albicans was verified; α-pinene presented an MIC between 128-512 µg/mL. In this study, we evaluate time-kill, a mechanism of action using in silico and in vitro tests, anti-biofilm activity against the Candida albicans, and toxicity against human cells (HaCaT). Results from the molecular-docking simulation demonstrated that thymidylate synthase (-52 kcal mol^-1), and δ-14-sterol reductase (-44 kcal mol^-1) presented the best interactions. Our in vitro results suggest that α-pinene's antifungal

SUBMITTER: Bomfim de Barros D 

PROVIDER: S-EPMC10044001 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature