Immunohistochemical visualization of lymphatic vessels in human dura mater: methodological perspectives.

ABSTRACT:

Background

Despite greatly renewed interest concerning meningeal lymphatic function over recent years, the lymphatic structures of human dura mater have been less characterized. The available information derives exclusively from autopsy specimens. This study addressed methodological aspects of immunohistochemistry for visualization and characterization of lymphatic vessels in the dura of patients.

Methods

Dura biopsies were obtained from the right frontal region of the patients with idiopathic normal pressure hydrocephalus (iNPH) who underwent shunt surgery as part of treatment. The dura specimens were prepared using three different methods: Paraformaldehyde (PFA) 4% (Method #1), paraformaldehyde (PFA) 0.5% (Method #2), and freeze-fixation (Method #3). They were further exam

SUBMITTER: Vera Quesada CL 

PROVIDER: S-EPMC10044429 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature