Identification of Duplication Genotypes of the Feathering Rate Gene in Chicken by a Multiplex PCR Following Electrophoresis and/or Sanger Sequencing.

ABSTRACT: Sex-linked phenotypes of late feathering (LF) and early feathering (EF) are controlled by a pair of alleles K and k⁺. Autosexing based on the feathering rate is widely used in poultry production. It is reported that a tandem duplication of 176,324 base pairs linked to the K locus is responsible for LF expression and could be used as a molecular marker to detect LF chicken. So far, there is no genotyping method that can accurately and stably identify the LF homozygote and heterozygote in all chicken breeds. In the present study, a multiplex PCR test was developed to identify EF, LF homozygote, and heterozygote according to electrophoretic bands and the relative height of the peaks by Sanger sequencing. We tested 413 chickens of six native Chinese breeds with

SUBMITTER: Shen Q 

PROVIDER: S-EPMC10044632 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature