Dataset Information

Fungal-Mediated Silver Nanoparticle and Biochar Synergy against Colorectal Cancer Cells and Pathogenic Bacteria.

ABSTRACT:

Background

Silver nanoparticles (AgNPs) are attractive substrates for new medicinal treatments. Biochar is pyrolyzed biomass. Its porous architecture allows it to hold and gather minuscule particles, through which nanoparticles can accumulate in its porous structure. This study examined AgNPs' antibacterial and anticancer properties alone and combined with biochar.

Methods

The fungus Emericella dentata was responsible for biosynthesis of AgNPs. The characterization of AgNPs using STEM images and a Zetasizer was carried out. Accordingly, the antibacterial and antiproliferation activity of AgNPs and biochar was studied using MIC and MTT assays, respectively. To evaluate the antiangiogenic and anti-inflammatory effects of AgNPs with biochar, VEGF and cytokines including TNF alpha, IL-6 and IL-beta were tested using an ELISA assay.

Results

The size of the AgNPs ranged from 10 to 80 nm, with more than 70% of them being smaller than 40 nm. The combination of AgNPs and biochar enhanced the antibacterial activity against all tested bacteria. Furthermore, this combination showed antiproliferative properties against HT29 cancer cells with high selectivity to fibroblasts at low concentrations. AgNPs with biochar significantly reduced VEGF and proinflammatory cytokine expression levels.

Conclusions

Biochar and AgNPs may be novel treatments for bacteria and colorectal cancer cells, according to the current findings.

SUBMITTER: Alqaraleh M

PROVIDER: S-EPMC10044691 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature

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Publications

Fungal-Mediated Silver Nanoparticle and Biochar Synergy against Colorectal Cancer Cells and Pathogenic Bacteria.

Alqaraleh Moath M Khleifat Khaled M KM Abu Hajleh Maha N MN Farah Husni S HS Ahmed Khaled Abdul-Aziz KA

Antibiotics (Basel, Switzerland) 20230316 3

<h4>Background</h4>Silver nanoparticles (AgNPs) are attractive substrates for new medicinal treatments. Biochar is pyrolyzed biomass. Its porous architecture allows it to hold and gather minuscule particles, through which nanoparticles can accumulate in its porous structure. This study examined AgNPs' antibacterial and anticancer properties alone and combined with biochar.<h4>Methods</h4>The fungus <i>Emericella dentata</i> was responsible for biosynthesis of AgNPs. The characterization of AgNPs ...[more]

PMID: 36978464

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Project description:Indian biodiversity is a hub for medicinal plants. Extensive research has been carried out to select plants with numerous properties which can be used for human welfare. Present research is about Cymbopogon citratus, an economically valuable medicinal plant. In this study Cymbopogon citratus was elected as a subject plant over the five selected plants (Azadirachta indica, Plumeria obtuse, Sapindus mukorossi, Capsicum annuum and Phyllanthus emblica) on the basis of antibacterial effect against dominating pathogenic species of gram positive (Bacillus cereus, Bacillus licheniformis) and gram negative (Pseudomonas aeruginosa, Escherichia coli) bacteria. Further, bioactive agents behind antibacterial potential of Cymbopogon citratus was analyzed using analytical method (Phyto-chemical, FTIR, NMR and GC-MS). Due to the broad antimicrobial spectrum, silver nanoparticles have turned into a noteworthy decision for the improvement of new medication. Therefore, this investigation further elaborated in the development of Cymbopogon citratus silver nano-particles (CNPs). Antibacterial potential of CNPs examine in a range of C25-C150 (μg/ml) through minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) where, C25 (μg/ml) concentration of CNPs were recorded as the MIC for all bacterial species and C25 (μg/ml) and C50 (μg/ml) noted as the MBC for Pseudomonas aeruginosa, Escherichia coli and Bacillus cereus, Bacillus licheniformis, respectively. In agar disk diffusion assay of CNPs, maximum diameter of zone of inhibition was observed for C150 (μg/ml) concentration Bacillus cereus (20.12 ± 0.42), Bacillus licheniformis (22.34 ± 0.4), Pseudomonas aeruginosa (35.23 ± 0.46) and Escherichia coli (31.87 ± 0.24). Involvement of bioactive component as a reducing and capping agent can be confirmed through FTIR spectrum of CNPs. Moreover XRD, EDXRF and SEM showed crystalline and cuboidal nature of CNPs with ∼35 nm sizes. Prominently, cytotoxic analysis was conducted to understand the toxic effect of CNPs. This research highlights the potential of CNPs due to the bioactive components present in Cymbopogon citratus extract: Polyphenols (phenol; 1584.56 ± 16.32 mg/L, Flavanoids) and mixture of terpenoids (Citral, Myrcene, Farnesol, β-myrcene and β -Pinene).

Dataset Information

Fungal-Mediated Silver Nanoparticle and Biochar Synergy against Colorectal Cancer Cells and Pathogenic Bacteria.

Background

Methods

Results

Conclusions

Publications

Fungal-Mediated Silver Nanoparticle and Biochar Synergy against Colorectal Cancer Cells and Pathogenic Bacteria.

Similar Datasets

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