Whole-genome sequencing analysis of two heat-evolved Escherichia coli strains.

ABSTRACT:

Background

High temperatures cause a suite of problems for cells, including protein unfolding and aggregation; increased membrane fluidity; and changes in DNA supercoiling, RNA stability, transcription and translation. Consequently, enhanced thermotolerance can evolve through an unknown number of genetic mechanisms even in the simple model bacterium Escherichia coli. To date, each E. coli study exploring this question resulted in a different set of mutations. To understand the changes that can arise when an organism evolves to grow at higher temperatures, we sequenced and analyzed two previously described E. coli strains, BM28 and BM28 ΔlysU, that have been laboratory adapted to the highest E. coli growth temperature reported to date.

Results

We found three large deletions i

SUBMITTER: McGuire BE 

PROVIDER: S-EPMC10044804 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature