Immunoglobulin adsorption and film formation on mechanically wrinkled and crumpled surfaces at submonolayer coverage.

ABSTRACT: Understanding protein adsorption behavior on rough and wrinkled surfaces is vital to applications including biosensors and flexible biomedical devices. Despite this, there is a dearth of study on protein interaction with regularly undulating surface topographies, particularly in regions of negative curvature. Here we report nanoscale adsorption behavior of immunoglobulin M (IgM) and immunoglobulin G (IgG) on wrinkled and crumpled surfaces via atomic force microscopy (AFM). Hydrophilic plasma treated poly(dimethylsiloxane) (PDMS) wrinkles with varying dimensions exhibit higher surface coverage of IgM on wrinkle peaks over valleys. Negative curvature in the valleys is determined to reduce protein surface coverage based both on an increase in geometric hindrance on concave surfaces, an

SUBMITTER: Gole MT 

PROVIDER: S-EPMC10044874 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature