Expression of the H₂O₂ Biosensor roGFP-Tpx1.C160S in Fission and Budding Yeasts and Jurkat Cells to Compare Intracellular H₂O₂ Levels, Transmembrane Gradients, and Response to Metals.

ABSTRACT: Intracellular hydrogen peroxide (H₂O₂) levels can oscillate from low, physiological concentrations, to intermediate, signaling ones, and can participate in toxic reactions when overcoming certain thresholds. Fluorescent protein-based reporters to measure intracellular H₂O₂ have been developed in recent decades. In particular, the redox-sensitive green fluorescent protein (roGFP)-based proteins fused to peroxiredoxins are among the most sensitive H₂O₂ biosensors. Using fission yeast as a model system, we recently demonstrated that the gradient of extracellular-to-intracellular peroxides through the plasma membrane is around 300:1, and that the concentration of physiological H₂O₂ is in the low nanomolar r

SUBMITTER: de Cubas L 

PROVIDER: S-EPMC10045392 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature