Dataset Information

Characterization of the Lower Airways and Oral Microbiota in Healthy Young Persons in the Community.

ABSTRACT: Lower airway dysbiosis contributes to disease pathogenesis in respiratory diseases. However, little is known regarding the microbiota of lower airways or the oral cavity of healthy young persons. To address this gap, 25 healthy persons (24.3 ± 3.3 years; 52% females; no current smokers) underwent bronchoscopy during which bronchial brushing (BB) and bronchoalveolar lavage (BAL) fluid were collected. Prior to the procedure, an oral wash (OW) sample was also obtained. Microbiome analyses (16S rRNA locus) were performed (alpha- and beta-diversity, taxa annotations, and predicted functional metagenomic profiles) according to the airway compartment (BB, BAL, and OW). The greatest microbial richness was observed in OW and the lowest in BB (p < 0.001). Microbial communities differed significantly across compartments (p < 0.001), especially between BB and OW. Taxa analyses showed a significantly higher abundance of Firmicutes (BB: 32.7%; BAL: 31.4%) compared to OW (20.9%) (p < 0.001). Conversely, Proteobacteria predominated in OW (27.9%) as opposed to BB (7.0%) and BAL (12.5%) (p < 0.001), mostly due to a greater abundance of the bacteria in the Haemophilus genus in the OW (p < 0.001). The lower airway microbiota (BB and BAL) is significantly different from the OW microbiota in healthy young persons with respect to microbial diversity, taxa profiles, and predicted function.

SUBMITTER: Leitao Filho FS

PROVIDER: S-EPMC10045431 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature

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Characterization of the Lower Airways and Oral Microbiota in Healthy Young Persons in the Community.

Leitao Filho Fernando Sergio FS Monica Peters Carli C Sheel Andrew William AW Yang Julia J Nislow Corey C Lam Stephen S Leung Janice M JM Sin Don D DD

Biomedicines 20230310 3

Lower airway dysbiosis contributes to disease pathogenesis in respiratory diseases. However, little is known regarding the microbiota of lower airways or the oral cavity of healthy young persons. To address this gap, 25 healthy persons (24.3 ± 3.3 years; 52% females; no current smokers) underwent bronchoscopy during which bronchial brushing (BB) and bronchoalveolar lavage (BAL) fluid were collected. Prior to the procedure, an oral wash (OW) sample was also obtained. Microbiome analyses (16S rRNA ...[more]

PMID: 36979819

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Dataset Information

Characterization of the Lower Airways and Oral Microbiota in Healthy Young Persons in the Community.

Publications

Characterization of the Lower Airways and Oral Microbiota in Healthy Young Persons in the Community.

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