Enhanced Levels of Peroxisome-Derived H₂O₂ Do Not Induce Pexophagy but Impair Autophagic Flux in HEK-293 and HeLa Cells.

ABSTRACT: Peroxisomes are functionally specialized organelles that harbor multiple hydrogen peroxide (H₂O₂)-producing and -degrading enzymes. Given that this oxidant functions as a major redox signaling agent, peroxisomes have the intrinsic ability to mediate and modulate H₂O₂-driven processes, including autophagy. However, it remains unclear whether changes in peroxisomal H₂O₂ (po-H₂O₂) emission impact the autophagic process and to which extent peroxisomes with a disturbed H₂O₂ metabolism are selectively eliminated through a process called "pexophagy". To address these issues, we generated and validated HEK-293 and HeLa pexophagy reporter cell lines in which the production of po-H₂

SUBMITTER: Li H 

PROVIDER: S-EPMC10045779 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature