Cell-Based Sensors for the Detection of EGF and EGF-Stimulated Ca²⁺ Signaling.

ABSTRACT: Epidermal growth factor (EGF)-mediated activation of EGF receptors (EGFRs) has become an important target in drug development due to the implication of EGFR-mediated cellular signaling in cancer development. While various in vitro approaches are developed for monitoring EGF-EGFR interactions, they have several limitations. Herein, we describe a live cell-based sensor system that can be used to monitor the interaction of EGF and EGFR as well as the subsequent signaling events. The design of the EGF-detecting sensor cells is based on the split-intein-mediated conditional protein trans-cleavage reaction (CPC). CPC is triggered by the presence of the target (EGF) to activate a signal peptide that translocates the fluorescent cargo to the target cellular location (mitochondria). The developed s

SUBMITTER: Lee E 

PROVIDER: S-EPMC10045995 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature