Tutorial for Stopped-Flow Water Flux Measurements: Why a Report about "Ultrafast Water Permeation through Nanochannels with a Densely Fluorous Interior Surface" Is Flawed.

ABSTRACT: Millions of years of evolution have produced proteinaceous water channels (aquaporins) that combine perfect selectivity with a transport rate at the edge of the diffusion limit. However, Itoh et al. recently claimed in Science that artificial channels are 100 times faster and almost as selective. The published deflation kinetics of vesicles containing channels or channel elements indicate otherwise, since they do not demonstrate the facilitation of water transport. In an illustrated tutorial on the experimental basis of stopped-flow measurements, we point out flaws in data processing. In contrast to the assumption voiced in Science, individual vesicles cannot simultaneously shrink with two different kinetics. Moreover, vesicle deflation within the dead time of the instrument

SUBMITTER: Pfeffermann J 

PROVIDER: S-EPMC10046062 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature