Carrageenan from Gigartina skottsbergii: A Novel Molecular Probe to Detect SARS-CoV-2.

ABSTRACT: The COVID-19 pandemic has caused an unprecedented health and economic crisis, highlighting the importance of developing new molecular tools to monitor and detect SARS-CoV-2. Hence, this study proposed to employ the carrageenan extracted from Gigartina skottsbergii algae as a probe for SARS-CoV-2 virus binding capacity and potential use in molecular methods. G. skottsbergii specimens were collected in the Chilean subantarctic ecoregion, and the carrageenan was extracted -using a modified version of Webber's method-, characterized, and quantified. After 24 h of incubation with an inactivated viral suspension, the carrageenan's capacity to bind SARS-CoV-2 was tested. The probe-bound viral RNA was quantified using the reverse transcription and reverse transcription loop-mediated

SUBMITTER: Zank PD 

PROVIDER: S-EPMC10046870 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature