Human Papillomavirus E7 and p16^INK4a mRNA Multiplexed Quantification by a QuantiGene^TM Proof-of-Concept Assay Sensitively Detects Infection and Cervical Dysplasia Severity.

ABSTRACT:

Background

Persistent infection with human papillomavirus (HPV) can lead to cervical cancer (CxCa). During the progression to CxCa, the expression of HPV oncogenes E6 and E7 is upregulated. In turn, cellular proteins such as p16^INK4a are also modulated. The combined detection of HPV oncogenes and cellular biomarkers indicative for dysplasia could be informative and convey better specificity than the current HPV tests that cannot discriminate transient infection from dysplastic changes.

Methods

The QuantiGene^TM 2.0 Plex Assay platform was chosen for the effective multiplexing and quantitative detection of seven HPV-E7 mRNA targets (HPV6, 16, 18, 31, 45, 59, and 68) and the cellular mRNA of p16^INK4a as a biomarker for HPV-induced transformation

SUBMITTER: Skof AS 

PROVIDER: S-EPMC10047034 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature