Increased Yield of Extracellular Vesicles after Cytochalasin B Treatment and Vortexing.

ABSTRACT: Extracellular vesicles (EVs) are promising therapeutic instruments and vectors for therapeutics delivery. In order to increase the yield of EVs, a method of inducing EVs release using cytochalasin B is being actively developed. In this work, we compared the yield of naturally occurring extracellular vesicles and cytochalasin B-induced membrane vesicles (CIMVs) from mesenchymal stem cells (MSCs). In order to maintain accuracy in the comparative analysis, the same culture was used for the isolation of EVs and CIMVs: conditioned medium was used for EVs isolation and cells were harvested for CIMVs production. The pellets obtained after centrifugation 2300× g, 10,000× g and 100,000× g were analyzed using scanning electron microscopy analysis (SEM), flow cytometry, the bicin

SUBMITTER: Kurbangaleeva SV 

PROVIDER: S-EPMC10047179 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature