Identification of Myelin Basic Protein Proximity Interactome Using TurboID Labeling Proteomics.

ABSTRACT: Myelin basic protein (MBP) is one of the key structural elements of the myelin sheath and has autoantigenic properties in multiple sclerosis (MS). Its intracellular interaction network is still partially deconvoluted due to the unfolded structure, abnormally basic charge, and specific cellular localization. Here we used the fusion protein of MBP with TurboID, an engineered biotin ligase that uses ATP to convert biotin to reactive biotin-AMP that covalently attaches to nearby proteins, to determine MBP interactome. Despite evident benefits, the proximity labeling proteomics technique generates high background noise, especially in the case of proteins tending to semi-specific interactions. In order to recognize unique MBP partners, we additionally mapped protein interaction networks for deam

SUBMITTER: Smirnova EV 

PROVIDER: S-EPMC10047773 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature