Proteome and Ubiquitylome Analyses of Maize Endoplasmic Reticulum under Heat Stress.

ABSTRACT: High temperatures severely affect plant growth and pose a threat to global crop production. Heat causes the accumulation of misfolded proteins in the endoplasmic reticulum(ER), as well as triggering the heat-shock response (HSR) in the cytosol and the unfolded protein response (UPR) in the ER. Excessive misfolded proteins undergo further degradation through ER-associated degradation (ERAD). Although much research on the plant heat stress response has been conducted, the regulation of ER-localized proteins has not been well-studied thus far. We isolated the microsome fraction from heat-treated and untreated maize seedlings and performed proteome and ubiquitylome analyses. Of the 8306 total proteins detected in the proteomics analysis, 1675 proteins were significantly up-regulated and 708 pr

SUBMITTER: Gao C 

PROVIDER: S-EPMC10047965 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature